DNA

Part:BBa_K4747013:Design

Designed by: Liu Lantian   Group: iGEM23_SUSTech-MED   (2023-10-11)


aiiA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 520
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 520
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 520
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 520
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 555


Design Notes

for aiiA expression in PAO1


Source

The aiiA gene encodes an enzyme that hydrolyzes N-acylhomoserine lactones (AHLs), disrupting quorum sensing in Gram-negative bacteria.

References